Ley Lab

Ley Lab

"You don't cure the disease by only looking at the disease. Very often cures come out of left field." — Klaus Ley, M.D. // Professor and Division Head
Division of Inflammation Biology

Overview

Klaus Ley, M.D., and his team study inflammation as a defense reaction caused by tissue damage or injury, characterized by redness, heat, swelling, and pain. The primary objective of inflammation is to localize and eradicate the irritant and repair the surrounding tissue. For the survival of the host, inflammation is a necessary and beneficial process. The inflammatory response involves three major stages: first, dilation of capillaries to increase blood flow; second, microvascular structural changes and escape of plasma proteins from the bloodstream; and third, leukocyte transmigration through endothelium and accumulation at the site of injury.

The leukocyte adhesion cascade is a sequence of adhesion and activation events that ends with extravasation of the leukocyte, whereby the cell exerts its effects on the inflamed site. Dr Ley has investigated the roles of adhesion molecules in acute and chronic inflammation with the ultimate goal to develop methods to control inflammation. One application is in atherosclerosis, the disease of the vessel wall that underlies heart attacks and strokes. Dr. Ley’s team is working on developing a vaccine against atherosclerosis. They have developed a vaccine that is effective in mice and are now aiming at translating this to a vaccine for humans.

From The Lab

Aug 31, 2016

LJI researchers gain new understanding of how neutrophils latch onto vessel walls to protect from infection and clean up injured tissue

Aug 1, 2016 // WebMD

The future of vaccines

Jul 31, 2016 // Frankfurter Allgemeine Sonntagszeitung

Immun gegen den Herzinfarkt? (Immune against heart attack)

Jul 20, 2016 // San Diego Union Tribune

Antibodies could stop heart disease, study finds

Jun 30, 2016

Harvards Springer get $51.5M from pharmas, others, from latest startup

Ley Lab

Publications

Biorheology

Leukocyte arrest: biomechanics and molecular mechanisms of _2 integrin activation

2016-12
Fan Z, Ley K
Circ Res

CCR5+T-bet+FoxP3+ effector CD4 T cells drive atherosclerosis

2016-03
Li J, McArdle S, Gholami A, Kimura T, Wolf D, Gerhardt T, Miller J, Weber C, Ley K
Nat Rev Drug Discov

Integrin-based therapuetics: biological basis, clinical use and new drugs

2016-03
Ley K, Rivera-Nieves J, Sandborn WJ, Shattil S
J Immunol

G_i2 and G_i3 differentially regulate arrenst from flow and chemotaxis in mouse neutrophils

2016-03
Kuwano Y, Adler M, Zhang H, Groisman A, Ley K
Arterioscler Thromb Vasc Biol

2015 russell ross memorial lecture in vascular biology: protective autoimmunity in atherosclerosis

2016-03
Ley K
Blood

Gnb isoforms orchestrate a signaling pathway comprising Rac1, Plc_2, and Plc_3 leading to LFA-1 activation and neutrophil arrest in vivo

2016-01
Block H, Stadtmann A, Riad D, Rossaint J, Sohlbach C, Germena G, Wu D, Simon SI, Ley K, Zarbock A
Journal of Cell Science

SLAT promotes TCR-mediated, Rap1-dependent LFA-1 activation and adhesion through interaction of its PH domain with Rap1

2015-12
Côte M, Fos C, Canonigo-Balancio AJ, Ley K, Bécart S, Altman A
J Clin Cell Immunol

Macrophage polarization: decisions that affect health

2015-10
Mills CD, Harris RA, Ley K
Journal of Leukocyte Biology

Role of the endothelial surface layer in neutrophil recruitment

2015-10
Marki A, Esko JD, Pries AR, Ley K
Cellular and Molecular Life Sciences

Beyond vascular inflammation-recent advances in understanding atherosclerosis

2015-10
Wolf D, Zirlik A, Ley K
Cardiovascular Research

Monocyte trafficking across the vessel wall

2015-08
Gerhardt T, Ley K
Immunity

HGF guides T cells into the heart

2015-06
Wolf D, Li J, Ley K
Autoimmunity

Vaccination to modulate atherosclerosis

2015-05
Kimura T, Tse K, Sette A, Ley K
Gastroenterology

SAMP1/YitFc mice develop ileitis via loss of CCL21 and defects in dendritic cell migration

2015-04
Mikulski Z, Johnson R, Shaked I, Kim G, Nowyhed H, Goodman W, Chodaczek G, Pizarro TT, Cominelli F, Ley K
Journal of Experimental Medicine

Monocyte phenotypes: when local education counts

2015-04
Quintar AA, Hedrick CC, Ley K
Hepatology

Soluble CD163 is assocaited with noninvasive measures of liver fibrosis in hepatitis C virus- and hepatitis C virus/human immunodeficiency virus-infected woman

2015-02
Kuniholm MH, Hanna DB, Landay AL, Kaplan RC, Ley K
Journal of Biomedical Optics

Intravital live cell triggered imaging system reveals monocyte patrolling and macrophage migration in atherosclerotic arteries

2015-02
McArdle S, Chodaczek G, Ray N, Ley K
Frontiers in Immunology

Macrophages at the fork in the road to health or disease

2015-02
Mills CD, Lenz LL, Ley K
Arteriosclerosis Thrombosis and Vascular Biology

Lymphocyte migration into atherosclerotic plaque

2015-01
Li J, Ley K
Circulation Research

Waking up the stem cell niche: how hematopoietic stem cells generate inflammatory monocytes after stroke

2015-01
Wolf D, Ley K
Thrombosis and Haemostasis

Genetic deletion of platelet glycoprotein Ib alpha but not its extracellular domain protects from atherosclerosis

2014-12
Koltsova EK, Sundd P, Zarpellon A, Ouyang H, Mikulski Z, Zampolli A, Ruggeri ZM, Ley K
Thrombosis and Haemostasis

L-selectin deficiency decreases aortic B1a and Breg subsets and promotes atherosclerosis

2014-10
Gjurich BN, Taghavie-Moghadam PL, Ley K, Galkina EV
Journal of Experimental Medicine

Fueling the fire: src family kinases drive inflammation

2014-09
Ley K
F10000Research

The second touch hypothesis: T cell activation, homing and polarization

2014-08
Ley K
Arteriosclerosis Thrombosis and Vascular Biology

Macrophage inflammatory markers are associated with subclinical carotid artery disease in women with human immunodeficiency virus of hepatitis c virus infection

2014-05
Shaked I, Hanna DB, Gleißner C, Marsh B, Plants J, Tracy D, Anastos K, Cohen M, Golub ET, Karim R, Lazar J, Prasad V,…
Frontiers in Immunology

Arrest chemokines

2014-04
Ley K
Frontiers in Immunology

Atheroprotective vaccination with MHC-II restricted peptides from ApoB-100

2013-12
Tse K, Gonen A, Sidney J, Ouyang H, Witztum JL, Sette A, Tse H, Ley K
International Immunology

T cells in atherosclerosis

2013-11
Tse K, Tse H, Sidney J, Sette A, Ley K
Proceedings of the National Academy of Sciences of the United States of America

Dysregulated NOD2 predisposes SAMP1/YitFc mice to chronic intestinal inflammation

2013-10
Corridoni D, Kodani T, Todriguez-Palacios A, Pizarro TT, Xin W, Nickerson KP, McDonald C, Ley KF, Abbott DW, Cominelli F
Current Opinion in Lipidology

Myeloid cells in atherosclerosis: a delicate balance of anti-inflammatory and proinflammatory mechanisms

2013-10
Koltslova EK, Hedrick CC, Ley K
Blood

Leukocytes talking to VE-cadherin

2013-10
Ley K
Journal of Experimental Medicine

The PSGL-1-L-selectin signaling complex regulates neutrophil adhesion under flow

2013-10
Stadtmann A, Germena G, Block H, Boras M, Rossaint J, Sundd P, Lefort C, Fisher CI, Buscher K, Gelschefarth B,…
Circulation Research

Increased atherosclerotic lesion formation and vascular leukocyte accumulation in renal impairment are mediated by interleukin 17A

2013-09
Ge S, Hertel B, Koltsova EK, Sörensen-Zender I, Kielstein JT, Ley KF, Haller H, von Vietinghoff S
Nature

Bacterial colonization facturs control specificity and stability of the gut microbiora

2013-09
Lee SM, Donaldson GP, Mikulski Z, Boyajian S, Ley K, Mazmanian SK
Molecular Immunology

Neutrophil rolling at high shear: flattening, catch bond behavior, tethers and slings

2013-08
Sundd P, Pospieszalska MK, Ley K
Immunity

The autoimmunity-associated gene PTPN22 potentiates toll-like receptor-driven, type 1 interferon-dependent immunigy

2013-07
Wang Y, Stanford SM, Zhou W, Curtsinger JM, Mikulski Z, Shaheen ZR, Cheng G, Sawatzke K, Campbell AM, Auger JL, Bilgic…
PLoS One

Increased cholesterol content in gammadelta (γδ) T lymphocytes differentially reguates their activationIncreased cholesterol content in gammadelta (γδ) T lymphocytes differentially reguates their activation

2013-05
Cheng HY, Wu R, Gegre AK, Hanna RN, Smith DJ, Parks JS, Ley K, Hedrick CC
Immunology and Cell Biology

Quantitative dynamic footprinting microscopy

2013-04
Sundd P, Ley K
Journal of Clinical Investigation

Dynamic T cell-APC interactions sustain chronic inflammation in atherosclerosis

2012-09
Koltsova EK, Garcia Z, Chodaczek G, Landau M, McArdle S, Scott SR, von Vietinghoff S, Galkina E, Miller YI, Action ST,…
Gastroenterology

Interleukin-17 ssignaling in inflammatory cells, kupffer, and hepatic stellate cells exacerabtes liver fibrosis in mice

2012-09
Meng F, Wang K, Aoyama T, Grivennikov SI, Paik Y, Scholten D, Cong M, Iwaisako K, Liu X, Zhang M, Osterreicher CH,…
Critical Care

Eliminating or blocking 12/15-lipoxygenase reduces neutrophil recruitment in mouse models of acute lung injury

2012-09
Rossaint J, Nadler JL, Ley K, Zarbock A
Cell

Regulated accumulation of desmosterol integrates macrophage lipid metabolism and inflammatory responses

2012-09
Spann NJ, Garmire LX, McDonald JG, Myers DS, Milne SB, Shibata N, Reichart D, Fox JN, Shaked I, Heudobler D, Raetz CR,…
Blood

Inactivation of heparan sulfate 2-O-sulfotransferase accentuates neutrophil infiltration during acute inflammation in mice

2012-08
Axelsson J, Xu D, Kang BN, Nussbacher JK, Handel TM, Leyy K, Sriramarao P, Esko JD
Nature

Slings' enable neutrophil rolling at high shear

2012-08
Sundd P, Gutierrez E, Koltsova EK, Kuwano Y, Fukuda S, Pospieszalska MK, Groisman A, Ley K
Nature Chemical Biology

Global metabolic inhinitors of sialyl- and fucosyltransferases remodel the glycome

2012-07
Rillahan CD, Antonopoulos A, Lefort CT, Sonon R, Azadi P, Ley K, Dell A, Haslam SM, Paulson JC
European Heart Journal

Transforming growth factor-β: transforming plaque to stability

2012-07
Tse K, Ley K
Journal of Immunology

Severe impairment of leukocyte recruitment in ppGalNAcT-1 deficient mice

2012-06
Block H, Ley K, Zarbock A
Blood

Distinct roles for talin-1 and kindlin-3 in LFA-1 extension and affinity regulation

2012-05
Lefort CT, Rossaint J, Moser M, Petrich BG, Zarbock A, Monkley SJ, Critchley DR, Ginsberg MH, Fässler R, Ley K
Circulation Research

Protective role for myeloid specific KLF2 in atherosclerosis

2012-05
Shaked I, Ley K
Journal of Immunology

Neutrophilic granulocytes modulate invariant NKT cell function in mice and humans

2012-04
Wingender G, Hiss M, Engel I, Peukert K, Ley K, Haller H, Kronenberg M, von Vietinghoff S
Arteriosclerosis Thrombosis and Vascular Biology

Regulation of neutrophil function by adenosine

2012-04
Barletta KE, Ley K, Mehrad B
Journal of Immunology

Protein kinase C-θ is required for murine neutrophil recruitment and adhesion strengthening under flow

2012-04
Bertram A, Zhang H, von Vietinghoff S, de Pablo C, Haller H, Shushakova N, Ley K
Arteriosclerosis Thrombosis and Vascular Biology

Accelerated atherosclerosis in apoe-/- mice heterozygous for the insulin receptor and the insulin receptor substrate-1

2012-02
Galkina EV, Butcher M, Keller SR, Goff M, Bruce A, Pei H, Sarembock IJ, Sanders JM, Nagelin MH, Srinivasan S, Kulkarni…
Circulation Research

NR4A1 (Nur77) deletion polarizes macrophages toward an inflammatory phenotype and increases atherosclerosis

2012-02
Hanna RN, Shaked I, Hubbeling HG, Punt JA, Wu R, Herrley E, Zaugg C, Pei H, Geissmann F, Ley K, Hedrick CC
Circulation Research

B-cell aortic homing and atheroprotection depend on Id3

2012-01
Doran AC, Lipinski MJ, Oldham SN, Garmey JC, Campbell KA, Skaflen MD, Cutchins A, Lee DJ, Glover DK, Kelly KA, Galkina…
Inflammatory Bowel Diseases

SAMP1/YitFc mouse strain: A spontaneous model of Chron's disease-like ileitis

2011-12
Pizarro TT, Pastorelli L, Bamias G, Garg RR, Reuter RK, Mercado JR, Chieppa M, Arsenear KO, Ley K, Cominelli F
Blood

Leukocyte ligands for endothelial selectins: specialized glycoconjugates that mediate rolling and signaling under flow

2011-12
Zarbock A, Ley K, McEver RP, Hidalgo A
Trends in Immunology

How dendritic cells shape atherosclerosis

2011-11
Koltsova EK, Ley K
Blood

CD63 positions CD62P for rolling

2011-10
Ley K
Science Signaling

Small molecule-mediated activation of the integrin CD11b/CD18 reduces inflammatory disease

2011-09
Maiguel D, Faridi MH, Wei C, Kuwano Y, Balla KM, Hernandez D, Barth CJ, Lugo G, Donnelly M, Nayer A, Moita LF, Schurer…
Arteriosclerosis Thrombosis and Vascular Biology

Monocyte and macrophage dynamics during atherogenesis

2011-07
Ley K, Miller YI, Hedrick CC
Journal of Visualized Experiemnts

Flow cytometry analysis of immune cells within murine aortas

2011-07
Butcher MJ, Herre M, Ley K, Galkina E
European Journal of Immunology

Rap1a activation by CalDAG-GEFI and p38 MAPK is involved in E-selectin-dependent slow leukocyte rolling

2011-07
Stadtmann A, Brinkhaus L, Mueller H, Rossaint J, Bolomini-Vittori M, Bergmeier W, Van Aken H, Wagner Dd, Laudanna C,…
Microcirculation

Live cell imaging of paxillin in rolling neutrophils by dual-color quantitative dynamic footprinting

2011-07
Sundd P, Gutierrez E, Petrich BG, Ginsberg MH, Ley K
Blood

Adam 17-dependent shedding limits early neutrophil influx but does not alter early monocyte recruitment to inflammatory sites

2011-07
Tang J, Zarbock A, Gomez I, Wilson CL, Lefort CT, Stadtmann A, Bell B, Huang LC, Ley K, Raines EW
Archives of Biochemistry and Biophysics

Protein tyrosine kinases in neutrophil activation and recruitment

2011-07
Zarbock A, Ley K
Journal of the American College of Cardiology

Mycophenolate mofetil decreases atherosclerotic lesion size by depression of aortic T lymphocyte and IL-17-mediated macrophage accumulation

2011-05
von Vietinghoff S, Koltsova EK, Mestas J, Diehl CJ, Witztum JL, Ley K
Archivum Immunologiae et Therapiae Experimentalis

Protein kinase C isoforms in neutrophil adhesion and activation

2011-04
Bertram A, Ley K
Biophysical Journal

Cell protrusions and tethers: a unified approach

2011-04
Pospieszalska MK, Lasiecka I, Ley K
Autoimmunity

Prevention, but not cure of type 1 diabetes by FTY720 in NOD/LtJ mice despite effective modulation of blood T cells

2011-03
Morris MA, McDuffie M, Nadler JL, Ley K
Journal of Innate Immunity

Sequential immune responses: the weapons of immunity

2005-07
Mills CD, Ley K, Buchmann K, Canton J
Front Immunol

How mouse macrophages sense what is going on

2016-06
Ley K, Pramod AB, Croft M, Ravichandran KS, Ting JP
Nat Commun

Protection from spetic peritonitis by rapid neutrophil recruitment through omental high endothelial venules

2016-03
Nat Commun

Protection from spetic peritonitis by rapid neutrophil recruitment through omental high endothelial venules

2016-03
Nat Rev Drug Discov

Integrin-based therapuetics: biological basis, clinical use and new drugs

2016-03
Ley K, Rivera-Nieves J, Sandborn WJ, Shattil S
Journal of Innate Immunity

M1 and M2 microphages: the chicken and the egg of immunity

2016-09
Mills CD, Ley K
Circulation Research

Interleukin-27 receptor limits atherosclerosis in Ldlr-/- mice

2016-09
Koltsova EK, Kim G, Lloyd KM, Saris CJ, von Vietinghoff S, Kronenberg M, Ley K
Frontiers in Immunology

Neutrophil arrest by LFA-1 activation

2016-09
Lefort CT, Ley K
PLoS One

High refractive index silicone gels for simultaneous total internal reflection flourescence and traction force microscopy of adherent cells

2016-09
Gutierrez E, Tkachenko E, Bresser A, Sundd P, Ley K, Danuser G, Ginsberg MH, Groisman A
Biorheology

Biomechanics of leukocyte rolling

2016-09
Sundd P, Pospieszalska MK, Cheung LS, Konstantopoulos K, Ley K
Am J Respir Crit Care Med

Leukocyte adhesion deficiency IV. Monocyte integrin activation deficiency in cystic fibrosis

2016-05
Fan Z, Ley K
Sci Rep

Microfluidics-based side view flow chamber reveals tether-to0sling translation in rollin gneutrophils

2016-06
Marki A, Gutierrez E, Mikulski Z, Groisman A, Ley K
J Exp Med

Live cell imaging to understand monocyte, macrophage, and dendritic cell function in atherosclerosis

2016-06
McArdle S, Mikulski Z, Ley K

Principal Investigator

ley

Klaus Ley, M.D.

Professor and Division Head

Dr. Ley joined LIAI in 2007 as the founding Division Head of the Division of Inflammation Biology. Dr. Ley received his B.S. from Altkönigschule-Gymnasium, Kronberg, Germany in 1976. In 1982, he received his M.D. from the Julius-Maximilians-Universität, Würzburg, Germany. Dr. Ley began his postdoctoral training from 1983 to 1987 at the Freie Universität Berlin, Germany. From 1987 to 1989, Dr. Ley was a visiting research scientist at the University of California, San Diego and returned to Freie Universität Berlin until 1994, when he joined the faculty of the University of Virginia. From 2001-2007, he was director of the Robert M Berne Cardiovascular Research Center at the University of Virginia.

Lab Members

Christian Rydén

Graduate Student

MichaelAbadier

Michael Abadier

Postdoctoral Researcher

Biosketch:
In 2008, I graduated from the German University in Cairo with a B.Sc. degree in Pharmacy and Biotechnology. Then, I moved to the University of Ulm in Germany to perform my master’s thesis project and I obtained a M.Sc. degree in the beginning of 2011. In September 2014, I obtained my Ph.D. in Immunology from Theodor Kocher Institute, University of Bern, Switzerland. My research mainly focused on the mechanism of T cell extravasation across the blood-brain barrier. I began working as a postdoc in the Ley laboratory at La Jolla Institute for Allergy and Immunology in May 2015.

Research Focus:
My research is focused on how CD4+ T cells adhere and roll under high shear during inflammation. Rolling is primarily mediated by the interaction of endothelial P-selectin-to-P-selectin glycoprotein ligand (PSGL)-1 expressed in T cells. Under high shear, tethers and slings formed by T cells become essential to support rolling. I am interested in understanding how tethers and slings function in stabilizing T cell rolling and how their formation is regulated.

Career Goals:
I hope to implement the knowledge and skills acquired during basic science research toward a better understanding of human diseases.

PramodAkulaBala

Fnu Pramod Akula Bala, Ph.D,

Bioinformatician

Biosketch:
I did two years of post-doctoral training in France in the field of Neuropharmacology. Then, I moved to the US and worked as a computational biologist/post-doctoral researcher at the University of North Dakota.

Research Focus:
My research areas mainly include drug, biomarker and vaccine discovery for immunological disorders.

Career Goals:
Pursuing career in drug and pharmaceutical research.

Rebecka Bodare

Graduate Student

Konrad Buscher

Postdoctoral Researcher

Biosketch:
I graduated from medical school in 2010 (Berlin, Germany) and worked as resident in the Department of Internal Medicine (Nephrology, Rheumatology) until 2015, when I joined Klaus Ley’s lab at the La Jolla Institute as a postdoc.

Research focus:

I am interested in monocyte patrolling and macrophage polarization in disease. A technical focus is intravital microscopy to study leukocyte behaviour in vivo.

Erk Ehinger

ZhichaoFan

Zhichao Fan, Ph.D.

Postdoctoral Fellow

Biosketch:
I graduated from Soochow University, China in 2008 with a B.S. degree in Biotechnology. I then obtained my Ph.D. in Biomedical Optics and Chemical Biology from the Fudan University, China in 2013. I began working as a postdoc in the Ley laboratory at the La Jolla Institute for Allergy and Immunology in August 2013.
Research Focus: My research projects are focused on the mechanisms of leukocyte rolling and arrest during inflammation in the microcirculation. I am interested in understanding the mechanism of integrin activation happen underline leukocyte arrest. I exploited microfluidics with molecularly defined surfaces and high resolution three-color total internal reflection microscopy to imaging the molecular dynamics during leukocyte rolling and arrest under high resolution in vitro, and using intravital microscopy to study leukocyte rolling and arrest in vivo.

Career Goals:
I plan to pursue a career in scientific research focused on molecular mechanism involved in integrin activation, leukocyte adhesion and developing advanced optical imaging techniques.

TakayukiKimura

Takayuki Kimura, MD, Ph.D.

Postdoctoral Fellow

Biosketch:
I graduated from the University of Tokyo in 2006 with a M.D. degree. Then I started clinical work at the University of Tokyo Hospital and National Center for Global Health and Medicine. I obtained my Ph.D. in Medicine from the University of Tokyo in 2014. I began to work as a postdoc in the Ley laboratory at the La Jolla Institute for Allergy and Immunology in May 2014.

Research Focus:
My research projects are focused on the roles of T cell immunity in atherosclerosis. I am interested in understanding how T cell-mediated immune response plays a role in pathogenesis of atherosclerosis, and in developing immunomodulatory treatment of atherosclerosis.

KoujiKobiyama

Kouji Kobiyama

Postdoctoral Fellow

Biosketch:
I graduated from Tokyo University of Pharmacy and Life Science in 2003. I obtained my Ph.D. from Yokohama City University Graduate School of Medicine in 2009. I started postdoctoral work in the Dr. Horii’s laboratory at the Research Institute for Microbial Diseases, Osaka University from April 2009 to March 2010. I then worked as a staff scientist in the Laboratory of Adjuvant Innovation at the National Institutes of Biomedical Innovation, Health and Nutrition until August 2015 (Dr. Ishii’s laboratory). I began working as a postdoc in the Dr. Ley laboratory at the La Jolla Institute for Allergy & Immunology in September 2015.

Research Focus:
My research interests focus on innate immunity and vaccine adjuvant. My research project is the development and optimization of non-communicable diseases vaccine especially atherosclerosis using several adjuvants. I want to know which kind of adjuvant is required and which type of immune responses should be regulated by adjuvant for atherosclerosis vaccine.

Career Goals:
I plan to pursue a career in scientific research focused on vaccine adjuvant to develop concepts of effective and safety vaccine against infectious and non-communicable diseases.

Goutam Krish

Intern

Mike Lee

Visiting Scientist

AlexMarki

Alex Marki

Postdoctoral Fellow

SaraMcArdle

Sara McArdle

Biosketch:
I graduated from Columbia University in 2009 with a B.S. in Biomedical Engineering. I performed my graduate research in the Ley laboratory, focusing on intravital microscopy of atherosclerotic arteries. In 2015, I obtained my PhD from the University of California, San Diego in Bioengineering, with a specialization in multi-scale biology. Since then I have worked at LJI in the microscopy core while continuing my work in atherosclerosis in the Ley laboratory.

Research Focus:
My research focuses on myeloid cell phenotype and function in atherosclerosis. I am using intravital microscopy, flow cytometry, and RNA-seq to define and characterize subsets of macrophages and dendritic cells found in atherosclerotic plaques.
Career goals: I plan to pursue a career in engineering and tool development for biomedical research.

JacquelineMiller

Jacqueline Miller, B.Sc

Lab Manager

Biosketch:
I graduated from the University of California San Diego in 2010 with a B.S. degree in Bioengineering: Pre-medical. I began working as a Research Associate in the Von Herrath laboratory at the La Jolla Institute for Allergy and Immunology in March 2011 and was promoted to the Research Associate II. In August 2013, I accepted my current position in the Ley laboratory as lab and mouse colony manager.

Research Focus:
The research projects I assist with focus on the roles of myeloid and T cells in atherosclerosis. I am interested in understanding the mechanisms and cellular players responsible for plaque formation and how immune tolerance can be medicated through vaccination in the future treatment of heart disease.

Career Goals:
I plan to pursue a career in clinical scientific research after obtaining a medical degree.

Leo Nicolai

Graduate Student

Joshua Rychak

Visiting Scientist

Maximilian Schell

Graduate Student

Xiaoyu Shi

Graduate Student

Biosketch:
Xiaoyu is a graduate student in the Ley Lab.

Dan Smith

Visiting Scientist

Hao Sun

Visiting Scientist

MelanieVassallo

Melanie Vassallo, BS

Flow Cytometry Specialist I

Biosketch:
I graduated from James Madison University in 2014 with B.S. degrees in both Biology and Psychology. While at JMU, I did 2 years of Behavioral Neuroscience and Psychopharmacology research. I then worked in clinical settings for a year before I moved across the country to begin working at the La Jolla Institute for Allergy and Immunology. I started working in the Ley Lab as a Flow Cytometry Specialist in January 2016.

Research Focus:

The projects I am working on in the Ley Lab are focused on obtaining a greater understanding of the underlying immune mechanisms involved in Atherosclerosis, and developing potential treatments to prevent it.

Career Goals:
I hope to pursue a career in medicine, and continue doing research in the realm of human disease.

Holger Winkels

Postdoctoral Fellow

DennisWolf

Dennis Wolf, MD

Postdoctoral Fellow

Biosketch:
I graduated from Medical School at the University of Freiburg, Germany, in 2007 and worked as a research assistant at the BakerIDI Heart and Diabetes Institute, Melbourne, from 2007 to 2008. I obtained my MD in 2011 at the University of Freiburg for my work on inflammatory leukocyte recruitment in atherosclerosis. In 2009 I started as clinical resident in cardiology at the University Heart Center in Freiburg and obtained my certificate in internal medicine. In 2014 I started as a postdoctoral fellow at the La Jolla Institute for Allergy and Immunology in October 2014.

Research Focus:
My research interest focusses on inflammatory and immune mechanisms in cardio-metabolic disease, such as in atherosclerosis and the metabolic syndrome. In particular, I am interested in understanding how immune cells and their effector functions contribute to disease initiation and progression. Currently, I am working on the adaptive immune response in atherosclerosis to understand how particular antigens drive a beneficial or harmful T-helper cell response.

Career Goals:
I plan to pursue a career as physician-scientist with a focus on clinical cardiology and on basic research in human cardiovascular disease.

Ley Lab

Research Projects

Neutrophil adhesion and activation

Atherosclerosis vaccine

Inflammation in atherosclerosis