Mehdi Benkahla, Ph.D.

Can a virus trigger type 1 diabetes?

FUNDED BY: the generosity of the donors to the Sierra Shapery Memorial

Type 1 diabetes (T1D) is a chronic disease where insulin-producing beta-cells in the pancreas are the victims of the body’s own immune cells. Beta-cells are responsible for insulin production and regulation of glucose balance. Patients with T1D require a lifelong insulin therapy and are at high risk of developing severe secondary complications. The incidence of T1D has been increasing over the years and this can only be explained by changes in the environment.

Decades of research have provided epidemiological evidence for an association between T1D and viruses particularly coxsackieviruses of the Enterovirus genus. The viruses’ implication in the crime is still not clear. They could either directly infect and kill beta-cells or indirectly by promoting an inflammatory environment attracting autoreactive T cells to the crime scene.

I will be using a newly developed model of human pancreatic slices (HPS), which can be kept alive in vitro. Pancreatic tissue is sliced 120 µm thick (slightly thicker than a human hair), which preserves endocrine, exocrine, innervation and immune tissue compartments. A coxsackievirus B3 genetically engineered to express an enhanced green fluorescent protein will be used to infect the HPS and address the following questions: Which cell types in the pancreas are susceptible to infection? What is the impact of viral infection on beta cell function? Which inflammatory responses are induced and do they correlate with beta cell function? What is the effect of viral infection on islet-resident macrophages and other immune interactions? What is the effect of viral infection on HLA class I expression and autophagy in islet pancreatic cells?

SPARKing Impact: This effort will generate a better understanding of the consequences of viral infections in the pancreas and its role in the onset of T1D.