Simon Brunel, Ph.D.

Can we cure autoimmune diseases with a single therapy?

FUNDED BY: the generosity of The Rosemary Kraemer Raitt FoundationTrust, John and Cim Kraemer and 2020 Various Donors

Autoimmune diseases such as psoriasis, scleroderma, multiple sclerosis, diabetes or immune disorders such as allergies are due to a misguided or disproportionate immune reaction. Autoimmune disorders are sharply on the rise in modern society particularly affecting women. Available treatments consist of daily or emergency medications. Therefore, I propose a novel approach that re-edits the immune cells involved in the autoimmune reaction, targeting autoimmune diseases at their root.

The main culprit in most autoimmune disease are autoreactive T cells, which recognize and destroy cells in the body. T cells are generated and educated in the thymus, a small organ in front of the heart, before they travel throughout the body. Our goal is to prevent autoreactive T cells from leaving the thymus.

Recently, it has been shown that a subset of immune cells, so called plasmacytoid dendritic cells (pDCs), are able, under certain conditions, to migrate to the thymus and trigger the death of the autoreactive T cells. They are doing so by capturing and displaying fragments of tissue proteins on their surface. When these “loaded” dendritic cells travel to the thymus and they trigger the self-destruction of any immune cells that recognize that particular tissue protein, thus eliminating autoreactive T cells.

The purpose of this project is to mimic this physiological elimination process by generating pDC that express tissue proteins and can find their way to the thymus in a mouse model of type 1 diabetes. In mice just as in humans with type 1 diabetes, autoreactive T cells recognize insulin and destroy the cells that produce it. In healthy people, trace amounts of insulin are present in the thymus helping to build a natural immune tolerance of insulin. I will inject modified pDCs that carry insulin into the thymus of NOD mice, which spontaneously develop type 1 diabetes, to build this immune tolerance and prevent the development of diabetes.

SPARKing Impact: If this novel therapeutic strategy works, it could represent a universal therapeutic approach for any autoimmune diseases.