Human Immune Profiling Consortium

The aim of this work, in collaboration with Bjoern Peters, Ph.D., and Pandurangan Vijayanand, M.D., Ph.D., is to characterize the immune response to dengue virus (DENV) and Mycobacterium tuberculosis (MTB), both of which represent major global health challenges. Specifically, the consortium will apply unbiased methods to identify T cell signatures associated with protection from or susceptibility to the two diseases. This open-ended approach relies heavily on RNA sequencing, a cutting-edge technique to comprehensively identify genes whose expression is enhanced or diminished by natural exposure and vaccination.

Selected References

Tian Y, Seumois G, De-Oliveira-Pinto LM, Mateus J, Herrera-de la Mata S, Kim C, Hinz D, Goonawardhana NDS, de Silva AD, Premawansa S, Premawansa G, Wijewickrama A, Balmaseda A, Grifoni A, Vijayanand P, Harris E, Peters B, Sette A, Weiskopf D. Molecular Signatures of Dengue Virus-Specific IL-10/IFN-γ Co-producing CD4 T Cells and Their Association with Dengue Disease. Cell Rep. 2019 Dec 24;29(13):4482-4495.e4. doi: 10.1016/j.celrep.2019.11.098. PubMed PMID: 31875555; PubMed Central PMCID: PMC6942518.

Tian Y, Babor M, Lane J, Seumois G, Liang S, Goonawardhana NDS, De Silva AD, Phillips EJ, Mallal SA, da Silva Antunes R, Grifoni A, Vijayanand P, Weiskopf D, Peters B, Sette A. Dengue-specific CD8+ T cell subsets display specialized transcriptomic and TCR profiles. J Clin Invest. 2019 Mar 18;130:1727-1741. doi: 10.1172/JCI123726. eCollection 2019 Mar 18. PubMed PMID: 30882366; PubMed Central PMCID: PMC6436856.

Grifoni A, Costa-Ramos P, Pham J, Tian Y, Rosales SL, Seumois G, Sidney J, de Silva AD, Premkumar L, Collins MH, Stone M, Norris PJ, Romero CME, Durbin A, Ricciardi MJ, Ledgerwood JE, de Silva AM, Busch M, Peters B, Vijayanand P, Harris E, Falconar AK, Kallas E, Weiskopf D, Sette A. Cutting Edge: Transcriptional Profiling Reveals Multifunctional and Cytotoxic Antiviral Responses of Zika Virus-Specific CD8(+) T Cells. J Immunol. 2018 Dec 15;201(12):3487-3491. doi: 10.4049/jimmunol.1801090. Epub 2018 Nov 9. PubMed PMID: 30413672; PubMed Central PMCID: PMC6287102.

Li S, Sullivan NL, Rouphael N, Yu T, Banton S, Maddur MS, McCausland M, Chiu C, Canniff J, Dubey S, Liu K, Tran V, Hagan T, Duraisingham S, Wieland A, Mehta AK, Whitaker JA, Subramaniam S, Jones DP, Sette A, Vora K, Weinberg A, Mulligan MJ, Nakaya HI, Levin M, Ahmed R, Pulendran B. Metabolic Phenotypes of Response to Vaccination in Humans. Cell. 2017 May 18;169(5):862-877.e17. doi: 10.1016/j.cell.2017.04.026. Epub 2017 May 11. PubMed PMID: 28502771; PubMed Central PMCID: PMC5711477.

Arlehamn CL, Seumois G, Gerasimova A, Huang C, Fu Z, Yue X, Sette A, Vijayanand P, Peters B. Transcriptional profile of tuberculosis antigen-specific T cells reveals novel multifunctional features. J Immunol. 2014 Sep;193(6):2931-40.