Support

Overview

Beginning Monday, May 11 2020, the Sequencing Core will be operating in Expanded Research Mode. Please find more info here: Sequencing Core Expanded Research Mode Guidelines

High-throughput sequencing is an extremely fast developing technology that has profoundly altered our understanding of biology, human diversity, and disease.

Services

Please acknowledge the use of the Sequencing Core Facility at the La Jolla Institute in publications. In addition, the NovaSeq 6000 and the HiSeq 2500 were acquired through the Shared Instrumentation Grant (SIG) Program (S10) and must be cited for their use: NovaSeq 6000 S10OD025052 and HiSeq 2500 S10OD016262.

Instrumentation

Illumina Miseq

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Agilent 2200 Tapestation

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Covaris Model E220

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Beckman Coulter Biomek FXP

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NovaSeq 6000

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Rates

Internal / Collaborators 3rd party non-profit 3rd party for-profit
Technical Training – Sequencing and Library Prep Specialist (per hour) $66.47 $72.45 $119.70
Technical Assistance (per hour) $44.31 $48.30 $79.80
Internal / Collaborators 3rd party non-profit 3rd party for-profit
Qiagen Full Service RNA Extraction (Trizol, per 12 samples)  $354.48  $386.40  $638.40
Qiagen Instrument Fee per 12 samples (does not include reagents  n/a  n/a  n/a
Tapestation Self-Service Full Tape (after Core training, reagents not supplied)  n/a  n/a  n/a
Tapestation HS Screen Tape (15 samples + ladder, full service)  $163.53  $178.25  $294.50
Tapestation (non-HS) Screen Tape (15 samples + ladder, full service)  $131.88  $143.75  $237.50
Tapestation RNA Screen Tape (15 samples + ladder, full service)  $141.37  $154.10  $254.60
Qubit Quantification 8 samples – Self Service (does not include reagents)  n/a  n/a  n/a
Qubit Quantification 8 samples – Full Service (includes reagents)  $36.00  $39.24  $64.84
Picogreen Quantification 96 Plate – Full Service  $89.45  $97.51  $161.10
Bead cleanup, Tapestation and Qubit QC, single sided  $62.70  $68.34  $112.92
Bead cleanup, Tapestation and Qubit QC, double sided  $86.13  $93.88  $155.11
Library Pool QC (Qubit, Tapestation, qPCR – Required for every partial FC submission, per library/pool)  $39.04  $42.55  $70.30
Library pooling, per sample up to 16 samples (includes fluorescence-based quant)  $15.83  $17.25  $28.50
Library pooling high/low samples, per sample (includes normalization and fluroescence-based quant)  $26.38  $28.75  $47.50
Internal / Collaborators 3rd party non-profit 3rd party for-profit
Illumina Stranded mRNA-seq Library Prep (Each sample, first 16 samples)  $179.35  $195.50  $323.00
Illumina Stranded mRNA-seq Library Prep (Each samples, 17-96 in plate)  $153.61  $167.44  $276.65
Ultra low input RNA-seq (using SMARTer Stranded methods) (Each sample, first 16 samples)  $288.02  $313.95  $518.70
Ultra low input RNA-seq (using SMARTer Stranded methods) (Each sample, samples 17-96)  $201.61  $219.77  $363.09
DNA-seq Library Prep (Each sample, first 16 samples)  $139.02  $151.54  $250.37
DNA-seq Library Prep (Samples 17-96)  $88.00  $95.92  $158.48
PCR-free DNA-seq Library Prep Add-on, per sample  $83.08  $90.56  $149.63
10X Genomics Single Cell RNA GEX 3′ Library Prep (each sample)  $2,049.34  $2,233.88  $3,690.75
10X Assay day (2 days per 3′ workflow)  $564.95  $615.83  $1,017.45
Additional 10X Chip  $332.33  $362.25  $598.50
10X Cell processing  $221.55  $241.50  $399.00
Other 10X Assays
Internal / Collaborators 3rd party non-profit 3rd party for-profit
MiSeq Self Service (nano; after Core training, does not include reagents)  $168.80  $184.00  $304.00
MiSeq Self Service (v2, v3; after Core training, does not include reagents)  $337.60  $368.00  $608.00
MiSeq v2 Nano Kit 300 Cycle Full Flowcell  $887.20  $967.09  $1,597.80
MiSeq v2 Nano Kit 500 Cycle Full Flowcell  $962.75  $1,049.44  $1,733.85
Miseq v2 50 Cycle Full Flowcell  $1,584.19  $1,726.85  $2,853.05
Miseq v2 300 Cycle Full Flowcell  $1,891.26  $2,061.56  $3,406.06
Miseq v2 500 Cycle Full Flowcell  $2,060.64  $2,246.19  $3,711.10
MiSeq v3 150 Cycle Full Flowcell  $1,703.61  $1,857.01  $3,068.11
MiSeq v3 600 Cycle Full Flowcell  $2,538.30  $2,766.87  $4,571.34
Internal / Collaborators 3rd party non-profit 3rd party for-profit
SP 100 Cycle Full Flowcell  $3,273.85  $3,568.65  $5,896.03
SP 200 Cycle Full Flowcell  $4,150.58  $4,524.33  $7,474.98
SP 300 Cycle Full Flowcell  $4,453.59  $4,854.63  $8,020.69
SP 500 Cycle Full Flowcell  $5,957.03  $6,493.45  $10,728.30
S1 100 Cycle Full Flowcell  $6,179.47  $6,735.91  $11,128.90
S1 200 Cycle Full Flowcell  $7,435.93  $8,105.52  $13,391.72
S1 300 Cycle Full Flowcell  $8,016.22  $8,738.06  $14,436.80
S2 100 Cycle Full Flowcell  $10,543.54  $11,492.96  $18,988.36
S2 200 Cycle Full Flowcell  $12,893.45  $14,054.47  $23,220.42
S2 300 Cycle Full Flowcell  $13,850.23  $15,097.40  $24,943.53
S4 35 Cycle Full Flowcell  $16,352.50  $17,825.00  $29,450.00
S4 200 Cycle Full Flowcell  $21,154.64  $23,059.57  $38,098.41
S4 300 Cycle Full Flowcell  $23,543.09  $25,663.08  $42,399.88
XP Workflow; 2 Lane  $397.57  $433.37  $716.01
XP Workflow; 4 Lane  $1,272.69  $1,387.30  $2,292.06

For more information, please visit the Bioinformatics page.

Internal / Collaborators 3rd party non-profit 3rd party for-profit
Programming or Technical Support (per hour)  $121.85  $132.83  $219.45
Mapping (per hour)  $288.02  $313.95  $518.70
Differential Expression Analysis  $221.55  $241.50  $399.00
NGS FASTQ and QC  $88.62  $96.60  $159.60
Internal / Collaborators 3rd party non-profit 3rd party for-profit
shRNA Cherrypick (16 or less)  $110.25  $121.28  $211.85
shRNA Cherrypick (17-96)  $330.75  $363.30  $635.53
siRNA Cherrypick (up to 96 wells)  $330.75  $363.83  $635.53
siRNA Cherrypick (up to 384 well)  $936.60  $1,030.84  $1,800.69