Support

Overview

Beginning Monday, May 11 2020, the Microscopy Core will be operating in Expanded Research Mode. Please find more info here: Microscopy Expanded Research Mode Guidelines

The Microscopy and Histology Core Facilities at La Jolla Institute were developed to help local scientists with their imaging needs in a static or dynamic (in vitro/in vivo) scenario by offering histology and microscopy services and providing support and training to interested researchers. Dedicated personnel can assist in all essential steps of an imaging experiment starting from experimental design, sample preparation through image acquisition and data analysis. The facility offers a range of light microscopes, related imaging systems and image analysis resources for assisted or independent use (upon training). The facility operates on a fee for use basis and is open to all potential users.

Services

From whole animal imaging to resolving details beyond the diffraction limit, LJI microscopy facility offers one stop shop for all your light microscopy needs. In addition to microscopy services, our staff offers full service histology. Dedicated personnel will take care of your samples to deliver high quality sections which can be digitized and analyzed with the help of our in-house experts. We offer advice on proper sample fixation, processing, embedding of tissues with attention to desired orientation, decalcification of bone tissue, cutting of paraffin blocks, cryosectioning, routine, special and immunostains as well as training in histological techniques.

Instrumentation

Leica SP8 Intravital Multiphoton/Confocal System

Location: BSL2 area with badge-restricted access View Specs

Zeiss LSM 880 AIRYSCAN FAST Confocal System

Location: Room 2113 (Microscopy Suite) View Specs

Zeiss LSM 780 Confocal System

Location: Room 2113 (Microscopy Suite) View Specs

Zeiss Axio Scan Z1 Slide Scanner

Location: Room 2328 (Histology Suite) View Specs

Nanoimager - Oxford Nanoimaging

Location: Room 2113 (Microscopy Suite) View Specs

Olympus FluoView FV10 I Confocal Microscope

Location: Room 2113 (Microscopy Suite) View Specs

Zeiss Axiovert 200M Marianas Deconvolution System

Location: Room 2113 (Microscopy Suite) View Specs

Nikon 80I Epifluorescence Microscope

Location: Room 2113 (Microscopy Suite) View Specs

Imaging Workstations

Location: Room 2113 (Microscopy Suite) View Specs

Histology Instrumentation

Location: View Specs

Rates

For all scheduling requests, please contact Zbigniew Mikulski in the form below.

Expand All
Internal/
Collaborators
3rd Party
non-profit
3rd Party
for-profit
Consultation $0.00 $80.85 $141.23
Training and assistance (added to the base price of the instrument) $55.13 $60.64 $105.92
Internal/
Collaborators
3rd Party
non-profit
3rd Party
for-profit
Imaging workstation (QuPath, Fiji, Imaris, ImagePro, ZEN, Slidebook) $5.51 $6.06 $10.59
Leica SP8 (confocal/multiphoton imaging) $33.08 $36.38 $63.55
Leica SP8 after hours 10pm-8am (confocal/multiphoton imaging) $16.54 $18.19 $31.78
LSM 880 with Airyscan $27.56 $30.32 $52.96
Zeiss LSM780 confocal $22.05 $24.26 $42.37
Zeiss LSM780 after hours, 10 pm – 8 am) $11.03 $12.13 $21.18
Zeiss AxioZ1 slide scanner $33.08 $36.38 $63.55
Nanoimager (per hour) $38.59 $42.45 $74.15
Olympus FV10i (confocal) $11.03 $12.13 $21.18
Nikon 80i (widefield, fluorescence and H&E staining) $5.51 $6.06 $10.59
Marianas Zeiss Axiovert 200M (widefield, fluorescence and DIC) $11.03 $12.13 $21.18
Ami HT $27.56 $30.32 $52.96
Data Storage (monthly per GB) $0/no charge $0.01 $0.02
Special Data Services varies per project varies per project varies per project
Internal/
Collaborators
3rd Party
non-profit
3rd Party
for-profit
Histology training $77.18 $84.89 $148.29
Grossing (Sample trimming) and cassetting (per hour) $33.08 $36.38 $63.55
Block with one HE slide (processing, embedding, cutting, and staining with HE) $7.72 $8.49 $14.83
Block Only (Includes all of the above except cutting) $3.31 $3.64 $6.36
Block with one (1) unstained slide $4.73 $5.20 $9.08
Extra unstained slide $1.42 $1.56 $2.72
Extra HE slide $3.05 $3.35 $5.85
Special Stain II (Stains for carbohydrates, connective tissue, muscle fibers, lipids, pigments, minerals, and nerve tissue per slide) $8.82 $9.70 $16.95
Special Stain III (Stains for microorganism, special cells using silver impregnation) $9.92 $10.91 $19.07
Frozen Sectioning $3.62 $3.98 $6.96
Assay handling, special project (per hour) $33.08 $36.38 $63.55
Decal $1.10 $1.21 $2.12
Microtome usage fee $5.25 $5.78 $10.09
Cryostate usage fee $5.25 $5.78 $10.09
Staining line $5.25 $5.78 $10.09
Self-service facility access fee (per lab, per month) $25.00 $27.50 $48.04
Internal/
Collaborators
3rd Party
non-profit
3rd Party
for-profit
Imaging supplies (high quality cover slips, dishes, etc.) varies per item varies per item varies per item
Imaging reagents (secondary antibodies, cell labeling reagents, per aliquot) $55.13 $60.64 $105.92
Histology supplies varies per item varies per item varies per item
Internal/
Collaborators
3rd Party
non-profit
3rd Party
for-profit
Filament Price (per gram) $0.05 $0.06 $0.10
Print Price (per gram) $0.53 $0.58 $1.01
Material Printing $0.53/hr + $52.50/kg $0.58/hr + $57.75/kg $1.02/hr + $100.88/kg
Training/hour $55.13 $60.64 $105.92
Project Support/Design (per hour) $33.08 $36.38 $63.55
Design Software (per hour) $5.51 $6.06 $10.59

 

Resources

Histology Resources on the Web
The Internet Pathology Laboratory
Histomorphologic Evaluation of Lymphoid Organs