Overview
The Microscopy and Histology Core Facilities at La Jolla Institute were developed to help local scientists with their imaging needs in a static or dynamic (in vitro/in vivo) scenario by offering histology and microscopy services and providing support and training to interested researchers. Dedicated personnel can assist in all essential steps of an imaging experiment starting from experimental design, sample preparation through image acquisition and data analysis. The facility offers a range of light microscopes, related imaging systems and image analysis resources for assisted or independent use (upon training). The facility operates on a fee for use basis and is open to all potential users.
Services
From whole animal imaging to resolving details beyond the diffraction limit, LJI microscopy facility offers one stop shop for all your light microscopy needs. In addition to microscopy services, our staff offers full service histology. Dedicated personnel will take care of your samples to deliver high quality sections which can be digitized and analyzed with the help of our in-house experts. We offer advice on proper sample fixation, processing, embedding of tissues with attention to desired orientation, decalcification of bone tissue, cutting of paraffin blocks, cryosectioning, routine, special and immunostains as well as training in histological techniques.
Core Members
Instrumentation
Leica SP8 Intravital Multiphoton/Confocal System
Zeiss LSM 880 AIRYSCAN FAST Confocal System
Zeiss LSM 780 Confocal System
Zeiss Axio Scan Z1 Slide Scanner
Nanoimager - Oxford Nanoimaging
Olympus FluoView FV10 I Confocal Microscope
Zeiss Axiovert 200M Marianas Deconvolution System
Nikon 80I Epifluorescence Microscope
Imaging Workstations
Histology Instrumentation
Rates
For all scheduling requests, please contact Zbigniew Mikulski in the form below.
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User Support
| InstituteAffiliate | 3rd Party non-profit |
3rd Party for-profit |
|
|---|---|---|---|
| Consultation | $68.30 | $90.00 | $240.00 |
| Training and assistance (added to the base price of the instrument) | $68.30 | $118.95 | $200.00 |
Microscopy Support Services
| InstituteAffiliate | 3rd Party non-profit |
3rd Party for-profit |
|
|---|---|---|---|
| Imaging workstation (QuPath, Fiji, Imaris, ImagePro, ZEN, Slidebook) | $6.08 | $6.69 | $11.68 |
| Leica SP8 (confocal/multiphoton imaging) | $36.50 | $40.16 | $70.10 |
| Leica SP8 after hours 10pm-8am (confocal/multiphoton imaging) | $18.25 | $20.08 | $35.05 |
| LSM 880 with Airyscan | $30.41 | $33.45 | $58.40 |
| Zeiss LSM780 confocal | $24.33 | $26.77 | $46.73 |
| Zeiss LSM780 after hours 10 pm – 8 am | $12.16 | $13.38 | $23.36 |
| Zeiss AxioZ1 slide scanner | $36.50 | $40.16 | $70.10 |
| Nanoimager (per hour) | $42.58 | $46.84 | $81.77 |
| Keyence | $12.17 | $13.39 | $23.37 |
| Olympus FV10i | $12.17 | $13.39 | $23.37 |
| Marianas Zeiss Axiovert 200M (widefield, fluorescence and DIC) | $12.17 | $13.39 | $23.37 |
| Ami HT | $30.41 | $33.45 | $58.40 |
| Data Storage (monthly per GB) | $0/no charge | Inquire for Rates | Inquire for Rates |
| Special Data Services | varies per project | varies per project | varies per project |
Histology Core Services
| InstituteAffiliate | 3rd Party non-profit |
3rd Party for-profit |
|
|---|---|---|---|
| Histology training | $89.32 | $155.55 | $261.54 |
| Grossing (Sample trimming) and cassetting (per hour) | $40.98 | $71.37 | $120.00 |
| Block with one HE slide (processing, embedding, cutting, and staining with HE) | $9.19 | $16.01 | $26.92 |
| Block Only (Includes all of the above except cutting) | $3.94 | $6.86 | $11.54 |
| Block with one (1) unstained slide | $5.62 | $9.79 | $16.46 |
| Extra unstained slide | $1.68 | $2.93 | $4.92 |
| Extra HE slide | $5.25 | $9.15 | $15.38 |
| Special Stain I (Stains for carbohydrates, connective tissue, muscle fibers, lipids, pigments, minerals, and nerve tissue per slide) | $12.19 | $21.23 | $35.69 |
| Special Stain II (Stains for microorganism, special cells using silver impregnation) | $13.14 | $22.88 | $38.46 |
| Frozen Sectioning | $4.31 | $7.50 | $12.62 |
| Assay handling, special project (per hour) | $40.98 | $71.37 | $120.00 |
| Decal | $15.76 | $27.45 | $46.15 |
| Decal | $3.15 | $5.49 | $9.23 |
Supplies
| InstituteAffiliate | 3rd Party non-profit |
3rd Party for-profit |
|
|---|---|---|---|
| Imaging supplies (high quality cover slips, dishes, etc.) | varies per item | varies per item | varies per item |
| Imaging reagents (secondary antibodies, cell labeling reagents, per aliquot) | varies per item | varies per item | varies per item |
| Histology supplies | varies per item | varies per item | varies per item |
3D Printing
| InstituteAffiliate | 3rd Party non-profit |
3rd Party for-profit |
|
|---|---|---|---|
| 3D Printing | varies per item | varies per item | varies per item |
| Training and assistance (per hour) | $68.30 | $91.98 | $200.00 |
| Project Support/Design (per hour) | $68.30 | $91.98 | $200.00 |