Support

Overview

Beginning Monday, May 11, 2020, the Flow Cytometry Core will be operating in Expanded Research Mode. Please find more info here: Flow Cytometry Core Facility Expanded Research Mode Guidelines

The LJI Flow Cytometry Core Facility is one of the largest flow cytometry cores in the San Diego area. The core facility is a full service flow cytometry laboratory that provides investigators with state-of-the-art equipment along with the necessary expertise and services to support cutting-edge research. The core facility offers a wide range of routine and custom flow cytometry, mass cytometry, cell sorting services, data analysis, strategic planning for novel assays, user training as well as full scale research collaborations. More than fee-for-service providers, flow cytometry core staff members are personally invested in the success of experiments and consider themselves temporary extensions of individual labs. The flow cytometry facility has extensive experience in daily instrument calibration and maintenance, multicolor panel design, immunophenotyping, intracellular and intranuclear staining.

Instrumentation

LSRII-1 / LSRII-2 / LSRII-3

Location: View Specs

LSR Fortessa

Location: View Specs

FACSCanto-II

Location: View Specs

Accuri C6

Location: View Specs

FACSCalibur

Location: View Specs

FACSAria-1 / FACSAria-2 / FACSAria-3 / FACSAria Fusion

Location: View Specs

CyTOF Helios Mass Cytometer

Location: View Specs

FlowJo Analysis Software

Location: View Specs

Cytobank Enterprise

Location: View Specs

Rates

For facility information and all training and scheduling requests, please contact the Flow Cytometry Core staff.

Expand All
Internal/
Collaborators
3rd Party
non-profit
3rd Party
for-profit
LSRII, LSR Fortessa or FACS Celesta (User-operated) $42.00 $46.20 $80.70
LSRII-3, FACS Canto-II (User-operated) $31.50 $34.65 $60.53
Accuri C6 (User-operated) n/a n/a n/a
Internal/
Collaborators
3rd Party
non-profit
3rd Party
for-profit
FACSArias (Staff-operated) $73.50 $80.85 $141.23
FACS Melody (Staff-operated) $73.50 $80.85 $141.23
FACS Arias or FACS Melody (User-operated) $63.00 $69.30 $121.05
Internal/
Collaborators
3rd Party
non-profit
3rd Party
for-profit
CyTOF Helios (Staff-operated) $105.00 $115.50 $269.01
CyTOF Helios (User-operated) $63.00 $69.30 $211.37
Internal/
Collaborators
3rd Party
non-profit
3rd Party
for-profit
Conjugation Reagent Kit (cost per reaction) $131.25 $144.38 $384.30
Custom Antibody Conjugation (cost per reaction – user provides purified antibody) $210.00 $231.00 $461.16
Antibody Bank Reagent – Antibody, Fluidigm (100 tests/cost per test) $5.25 $5.78 $15.37
Antibody Bank Reagent – Antibody, Fluidigm (50 tests/cost per test) $10.50 $11.55 $30.74
Antibody Bank Reagent – Common Core Stock, In-house conjugated (cost per test) $4.20 $4.62 $11.53
Barcoding Reagent (Pd conjugated, per sample) $52.50 $57.75 $192.15
Barcoding Reagent (Palladium, unconjugated) $105.00 $115.50 $384.30
Internal/
Collaborators
3rd Party
non-profit
3rd Party
for-profit
LSRII-1, LSRII-2, LSR Fortessa, FACSCelesta (session = charge for instrument use per hour) $42.00 $46.20 $80.70
LSRII-3, FACSCanto-II (session = charge for instrument use per hour) $31.50 $34.65 $60.53
Accuri C6, FACSCalibur (session = charge for instrument use per hour) n/a n/a n/a
FACSMelody (session = session for instrument use per hour) $63.00 $69.30 $121.05
Technical assistance $73.50 $80.85 $141.23

Resources

FluoroFinder – Panel Design Tool – A core resource used at leading flow cytometry research facilities. View Link
Multicolor Flow Cytometry View Link
BD – Intro to Flow Cytometry View Link
Invitrogen – Fluorescence Tutorials View Link
BD Fluorescence Spectrum Viewer View Link
Fluorescence SpectraViewer View Link
Flowjo – The Daily Dongle View Link
BD – Basics of Sorting View Link