Vijayanand Lab

Vijayanand Lab

— Pandurangan Vijayanand, M.D. Ph.D. // Associate Professor, Division of Vaccine Discovery
William K. Bowes Distinguished Professor

Overview

Dr. Vijayanand lab employs genomics tools to understand, diagnose and treat pulmonary disease such as asthma, lung cancer and infectious disease.

His laboratory has developed a number of techniques to study the molecular profiles of circulating and airway immune cells from patients with asthma and other diseases, using fewer cells than was possible previously.

Dr. Vijayanand also oversees a large-scale effort to map epigenomic modifications in more than a dozen different types of human immune cells from normal individuals to understand how epigenetic variations cause susceptibility to disease. For many human diseases, large-scale genomic studies have identified common genetic variants that occur more frequently in people with cardiovascular, autoimmune, inflammatory and infectious diseases, diabetes and asthma than in those without these diseases. However, it has been difficult to pinpoint which genetic changes are truly relevant to disease, or in what specific cell types the genetic variation has the largest effect on one’s health. Mapping the epigenome will allow Vijay and his team to zoom in on those genes with the greatest likelihood to contribute to disease, and the cell types in which they act, and therefore will help identify novel therapeutic targets.

From The Lab

Nov 15, 2018 // Cell

DICE: Immune cell atlas goes live

Jan 19, 2018 // Science Immunology

Novel genomic tools provide new insight into human immune system

Dec 6, 2017

San Diego Team wins $ 6.9 million grant to establish PrecISE Network Clinical Center

Jun 19, 2017

Tumor immune fitness determines survival of lung cancer patients

Oct 12, 2015

La Jolla Institute welcomes new faculty member

Vijayanand Lab

Publications

J Clin Invest

Th1/Th17 polarization persists following whole-cell pertussis vaccination dspite repeated acellular boosters

2018-08
da Silva Antunes R, Babor M, Carpenter C, Khalil N, Cortese M, Mentzer AJ, Seumois G, Petro CD, Purcell LA, Vijayanand…
Cell Rep

Identification of an early unipotent neutrophil progenitor with pro-tumoral activity in mouse and human bone marrow

2018-08
Zhu YP, Padgett L, Dinh HQ, Marcovecchio P, Blatchley A, Wu R, Ehinger E, Kim C, Mikulski Z, Seumois G, Madrigal A,…
J Immunol

Transcriptomic analysis of CD4+ T cells reveals novel immune signatures of latent tuberculosis

2018-05
J Allergy Clin Immunol

Allergen-specific immunotherapy modulates the balance of circulating Tfh and Tfr cells

2018-02
Sci Immunol

Precursors of human CD4+ cytotoxic T lymphocytes identified by single-cell transcriptome analysis

2018-01
Patil VS, Madrigal A, Schmiedel BJ, Clarke J, O'Rourke P, de Silva AD, Harris E, Peters B, Seumois G, Weiskopf D, Sette…
J Natl Cancer Inst

Targeting the myofibroblastic cancer-associated fibroblast phenotype through inhibition of NOX4

2018-01
Hanley CJ, Mellone M, Ford K, Thirdborough SM, Mellows T, Frampton SJ, Smith DM, Harden E, Szyndralewiez C, Bullock M,…
Methods Mol Biol

An integrated and semiautomated microscaled approach to profile cis-regulatory elements by histone modification ChIP-Seq for large-scale epigenetic studies

2018-01
Clin Cancer Res

Head and neck squamous cell carcinomas are characterized by a stable immune signature within the primary tumor over time and space

2017-12
Wood O, Clarke J, Woo J, Mirza AH, Woelk CH, Thomas GJ, Vijayanand P, King EV, Ottensmeier CH
Nat Commun

Unique phenotypes and clonal expansions of human CD4 effector memory T cell re-expressing CD45RA

2017-11
Tian Y, Babor M, Lane J, Schulten V, Patil VS, Seumois G, Rosales SL, Fu Z, Picarda G, Burel J, Zapardiel-Gonzalo J,…
Br J Cancer

Evaluating the effect of immune cells on the outcome of patients with mesothelioma

2017-10
Chee SJ, Lopez M, Mellows T, Gankande S, Moutasim KA, Harris S, Clarke J, Vijayanand P, Thomas GJ, Ottensmeier CH
Am J Respir Crit Care Med

Differential recognition of mycobacterium tuberculosis-specific epitopes as a function of tuberculosis disease history

2017-09
Scriba TJ, Carpenter C, Carrasco Pro S, Sidney J, Musvosvi M, Rozot V, Seumois G, Rosales SL, Vijayanand P, Goletti D,…
Nat Immunol

Tissue-resident memory features are linked to the magnitude of cytotoxic T cell responses in human lung cancer

2017-08
Ganesan AP, Clarke J, Wood O, Garrido-Martin EM, Chee SJ, Mellows T, Samaniego-Castruita D, Singh D, Seumois…
J Allergy Clin Immunol

Allergen-specific immunotherapy modulates the balance of circulating Tfh and Tfr cells

2017-05
Schulten V, Tripple V, Seumois G, Qian Y, Scheuemann RH, Fu Z, Locci M, Rosales S, Vijayanand P, Sette A, Alam R,…
J Immunol

An integrated workflow to assess technical and biological variability of cell population frequencies in human peripheral blood by flow cytometry

2017-02
Burel JG, Qian Y, Lindestam Arlehamn C, Weiskopf D, Zapardiel-Gonzalo J, Taplitz R, Gilman RH, Saito M, de Silva AD,…
Nature Communications

17q21 asthma-risk variants switch CTCF binding and regulate IL-2 production by T cells.

2016-11
Schmiedel BJ, Seumois G, Samaniego-Castruita D, (...) Sette A, Peters B, Vijayanand P*.
Oncotarget

Gene expression analysis of TIL rich HPV-driven head and neck tumors reveals a distinct B-cell signature when compared to HPV independent tumors.

2016-07
Wood O, Woo J, Seumois G, (...) Vijayanand P*, Woelk CH, King E, Ottensmeier C.
Journal of Experimental Medicine

Control of Foxp3 stability through modulation of TET activity.

2016-03
Yue X, Trifari S, Äijö T, Tsagaratou A, Pastor WA, Zepeda-Martínez JA, Lio CW, Li X, Huang Y, Vijayanand P*, …
Journal of Experimental Medicine

Identification of a novel cis-regulatory element essential for immune tolerance

2015-11
LaFlam TN, Seumois G, Miller CN, Lwin W, Fasano KJ, Waterfield M, Proekt I, Vijayanand P*, Anderson MS
Clin Exp Allergy

Lack of allergy to timothy grass pollen is not a passive phenomenon but associated with allergen-specific modulation of immune reactivity

2015-05
Hinz, D, Seumois, G, (...), Peters, B, Vijayanand, P*, Sette, A
Journal of Immunology

Transcriptional profile of tuberculosis antigen-specific T cells reveals novel multifunctional features

2014-09
Arlehamn, C.L., Seumois, G., Gerasimova, A., Huang, C., Fu, Z., Yue, X., Rao, A., Sette, A., Vijayanand, P*, Peters, B*
Nature Immunology

Epigenomic analysis of primary human T cells reveals novel enhancers associated with Th2 memory differentiation and asthma susceptibility

2014-08
Seumois, G, Chavez, L, Gerasimova, A, (...), Peters, B, Rao, A, Vijayanand, P*.
PLOS One

Predicting cell types and genetic variations contributing to disease by combining GWAS and epigenetic data

2013-01
Gerasimova, A, Chavez, L, Li, B, Seumois, G, Greenbaum, J, Rao, A, Vijayanand, P*, Peters, B.
Am J Clin Exp Immunol

An integrated nano-scale approach to profile miRNAs in limited clinical samples

2012-11
Seumois, G.*, Vijayanand, P*., Eisley, C.J., Omran, N., Kalinke, L., North, M., Ganesan, A.P., Simpson, L.J.,…
Immunity

Interleukin-4 Production by Follicular Helper T Cells Requires the Conserved Il4 Enhancer Hypersensitivity Site V.

2012-02
Vijayanand, P*, Seumois, G, Simpson, LJ, Abdul-Wajid, S, Baumjohann, D, Panduro, M, Huang, X, Interlandi, J, Djuretic,…
Thorax

Sputum IL-6 concentrations in severe asthma and its relationship with FEV1

2011-06
Morjaria, J.B., Babu, K.S., Vijayanand, P.*, Chauhan, A.J., Davies, D.E., and Holgate, S.T.
Journal of Immunology

Chemokine receptor 4 plays a key role in T cell recruitment into the airways of asthmatic patients

2010-04
Vijayanand, P.*, Durkin, K., Hartmann, G., Morjaria, J., Seumois, G., Staples, K.J., Hall, D., Bessant, C.,…
The New England Journal of Medicine

Invariant natural killer T cells in asthma and chronic obstructive pulmonary disease

2007-04
Vijayanand, P.*, Seumois, G., Pickard, C., Powell, R.M., Angco, G., Sammut, D., Gadola, S.D., Friedmann, P.S., and…

Principal Investigator

Pandurangan Vijayanand, M.D. Ph.D.

Associate Professor, Division of Vaccine Discovery

Dr. Vijayanand was appointed an Associate Professor in the Division of Vaccine Discovery in 2015. He is the inaugural holder of the William K. Bowes Distinguished Professorship

Dr. Vijayanand received his M.D. from the MGR Medical University in Chennai, India, and completed his residency in Internal Medicine followed by a Pulmonary Fellowship in the United Kingdom. In 2008, he obtained his Ph.D. from the University of Southampton, United Kingdom, where he studied the mechanisms of accumulation and activation of T cells in human asthma. Since then, Dr. Vijayanand has split his time between laboratory research and seeing patients at the University of Southampton, where he currently holds an appointment as an Associate Professor in the School of Medicine. In 2007, he was awarded a prestigious National Career Development Fellowship to undertake translational studies in the epigenetic regulation of the immune cell signaling molecules in human asthma at UC San Francisco, where he was appointed adjunct assistant professor in the Department of Medicine in 2009. Two years later, Vijay joined the La Jolla Institute for Immunology as an adjunct assistant professor in the Division of Signaling and Gene Expression.

Lab Members

Praveen Akuthota

Clinical Associate Professor

Dr. Akuthota’s research efforts range from basic scientific investigations of human eosinophil biology and eosinophilic inflammation to clinical and translational efforts in asthma and eosinophilic pulmonary disease. He is the corresponding Principal Investigator for the UCSD Clinical Center in the National Heart, Lung, and Blood Institute’s PrecISE Network that will be studying precision interventions in severe asthma. He is a co-investigator on a study published in the New England Journal of Medicine showing the efficacy of anti-IL-5 therapy in the treatment of the eosinophilic disease EGPA (Churg Strauss syndrome). His research group is involved in other multicenter studies in eosinophilic disease and asthma. He is a Clinical Affiliate at the La Jolla Institute for Allergy and Immunology, working with them in developing joint efforts in asthma research (www.togetherforasthma.org). Basic science investigations from Dr. Akuthota focus on ex vivo studies of human eosinophils and include published work studying eosinophil trafficking and work studying microparticle release by eosinophils. He has written several reviews and chapters discussing eosinophil immunobiology and clinical considerations in eosinophilic diseases. Dr. Akuthota is an At-Large Member of the Board of Directors of the International Eosinophil Society.

Sourya Bhattacharyya

Bioinformatics Postdoctoral Fellow

Biosketch:
I did the masters (M.S. in 2012) and Ph.D (in 2017) from the Department of Computer Science and Engineering, Indian Institute of Technology Kharagpur, India. Prior to that, I have also worked in STMicroelectronics India Pvt Ltd after undergrads (2006-2009). My office days and masters’ involved research in video, image and biomedical signal processing, the most common research domain a computer engineer (in India) chooses. Suddenly the willingness (and lots of force) from my PhD supervisor shifted my domain to bioinformatics. Being almost a lone warrior with a computer science background and without any collaboration, my PhD research focused on developing algorithms in Computational Phylogenetics. I wanted to explore real genetic data and models for disease specific analysis, with active collaboration from expert biologists. Thanks to LJI (specifically Ferhat and Vijay) for providing me such an opportunity since April 2017.

Research Focus:
I am working on the development of computational algorithms in the 3C (chromosome conformation capture) domain, mostly focusing on HiChIP data. I am also working on building computational pipelines for bioinformatic analysis of ChIP-seq and ATAC-seq datasets.

Career goals:

Want to explore NGS data towards disease specific genome regulation analysis, and develop various computational and analytical models.

Howard Brickner

Visiting Scientist

Justin Cayford

Visiting Scientist

Vivek Chandra

Instructor

Biosketch:
I did PhD from ICGEB, New Delhi component, India. My studies characterized several pathways modulated by the Hepatitis E Virus that are major contributors to viral pathogenesis. To study how lncRNA and chromatin biology is revolutionizing our understanding of complex biological problems, I joined the laboratory of Cornelis Murre at University of California, San Diego in September 2010 as a Postdoctoral fellow. Recently from September 2017, I joined Vijay’s group at LJI as an Instructor.

Research Focus & Career Goals:
Recent work, to which I have also contributed, and from some other laboratories has established that global regulation of cellular differentiation is the result of the coordinated interplay of dynamic changes in the organization of chromatin, epigenetic modifications, lncRNAs and transcription factors. I will continuously look for ways to incorporate new features into the construction of a more complete regulatory-network map. I am convinced that the growing molecular toolbox at our disposal necessitates a quantitative systems approach to answer biological problems. By comparing the regulatory networks of pathologic conditions, I expect to gain new insights into the central question: how do regulatory networks instruct cellular behavior under diseased condition and can we discover drugs that can alter these regulatory networks.

James Clarke

Postdoctoral Fellow

I graduated my combined mRES and PhD in cancer immunology from the labs of Dr Vijayanand and Dr Ottensmierer in 2018. Having spent three years split between the two labs. I am currently involved in projects applying high-resolution immune monitoring to better understand patient heterogeneity in human tumours. My career goals are to continue developing as a Wet and Dry lab scientist to better understand human immunology.

Damaris Diaz

Clinical Coordinator

Simon Eschweiler

Postdoctoral Fellow

Biosketch:
During my MSc in Molecular Biotechnology (2013 – RWTH Aachen, Germany) I found my calling in studying the immune system and hence went on to obtain my PhD in Immunology (2017 – University of Luebeck). During my graduate work, I investigated the regulatory pathway and microenvironmental cues that guide antibody glycosylation and placed special emphasis on the regulation of the germinal center reaction by stimulatory T follicular helper and suppressive T follicular regulatory cells. In late 2017 I joined Vijay’s lab to investigate tumor-infiltrating lymphocyte subsets and their contribution to an efficacious anti-tumor immune response.

Research Focus:
I am implementing my expertise into a specific disease-focused perspective in cancer research to elucidate pathways of tumor evasion and the identification of novel immunotherapy targets.

Career Goals:
Improve cancer treatment efficacy by unraveling novel drug targets and rationally combining existing immunotherapy drugs

Anusha Preethi Ganesan

MD, PhD

Biosketch:
I obtained my MD from Madras Medical College and completed my pediatric residency in the UK. Fascinated by the concept of cancer vaccines, I undertook my PhD in tumor immunology jointly at the University of Southampton (Ottensmeier Lab) and University of California, San Francisco (Coussens Lab). As part of the UCSD pediatric hematology oncology fellowship program, I am currently pursuing subspecialty training and postdoctoral research.

Research Focus:
The immune system plays a critical role in regulating tumor growth and progression. My research is focused on elucidating the molecular mechanisms underlying a protective anti-tumor immune response with the goal of identifying novel immunotherapeutic targets.

Career Goals:
As a physician scientist, my goal is to undertake translational research into pediatric cancers, particularly, relapsed leukemia and harness the immune system to provide durable immunity against this disease.

Ravindra Gujar

Postdoctoral Fellow

Biosketch:
I got my Masters degree in Biotechnology from Madurai Kamaraj University, India. Thereafter I did my PhD from Jawaharlal Nehru University, India. My Doctoral work focused on innate immunity in context to immunosuppressive receptors on Dendritic cells. Soon after completing my PhD, I was given the opportunity to join Vijay’s lab.

Research Focus & Career Goals
My primary focus was to develop low cell number Epigenetics assay like ATAC-seq or Chip-seq. I am investigating the origins of asthma by studying children from birth till they develop asthma later in life. I am working on various aspects in project which include genome sequencing, epigenetic assays, and functional studies in human immune cells and further validation of key molecule(s) in mouse asthma models. My goal is to become scientific researcher in understanding and unraveling molecular mechanism of Asthma.

Shu Liang

Lab Manager/Research Technician II

Biosketch:
I got my B.S. degree from Shanghai Jiao Tong University in Laboratory Medicine, then my M.S. from University of Washington in the same major. I did some web development work before I realized how much I like working in a lab. Lucky enough, I was given the opportunity to join Vijay’s lab.
I’ve been working on NGS library preparation since I joined, and got familiar with more aspects of NGS technology as I grow. After getting promoted as the lab manager, I took on more responsibilities on supporting the smooth performance of the whole lab.

Research Focus:
Currently, the majority of my work goes to NGS library preparation protocol optimization in two major ways: developing highly sensitive and cost-effective RNA sequencing protocols that are suitable for our lab’s research subjects; optimizing the protocols to increase the throughput.

Career Goal:
My interests lie in new technologies. I plan to become an assay or instrument developer that push the limits restraining scientists from achieving their research goals.

Ariel Madrigal

Research Technician II

Biosketch:
Born in Mexico City, I obtained my bachelor’s degree in Genomic Sciences (2017) from National Autonomous University of Mexico. This program combines different disciplines, mainly biology, computer science and mathematics. I joined Vijay’s lab in June 2016 as a research technician focused on analyzing transcriptomic and epigenomic data.

Research Focus:
My main focus is to understand immune cell populations through the development and employment of computational algorithms. I enjoy analyzing vast amounts of data derived from techniques such as single cell RNA-Seq, ChIP-Seq, ATAC-Seq, etc. I found fascinating how all these data allow us to comprehend better the function of a cell. Lately, I’ve been interested in understanding how genetic variation affects the phenotype of diverse cell populations, and how this translates into predisposition to disease.

Career goals:
My short-term goal is to continue my education obtaining a Ph.D. in Bioinformatics. In the future, I see myself as a researcher focused on understanding genomic regulation, especially the role of non-coding genetic variation in human disease.

Sara Herrera de la Mata

Research Technician I

Biosketch:
Born in Madrid, Spain, I obtained my B.S. Double Degree in Pharmacology (2016) and Biotechnology (2017) from the European University of Madrid in Spain. In 2016, I travelled to the USA to finish my degree at the University of California Riverside (UCR), where I also obtained a Postgraduate in Bioengineering Management and joined an on-campus laboratory working in immunology and cancer. In 2017, I had the great opportunity to join the Vijayanand Laboratory as a Research Technician focused on the development of high-throughput sequencing technologies such as single cell transcriptomic and RNA-seq, and being involved in some of the projects carried out in the laboratory, like asthma and infectious diseases. Joining Vijay´s Lab and La Jolla Institute for Immunology is being a great experience for me and I believe this opportunity is boosting and adding enormous value to my career development.
Research Focus:
Currently, I am involved in multiple projects performing highly complex genomics assays in human samples which consist of micro-scaled sample processing, preparation of sequencing libraries, the performance of sequencing runs, and the posterior analysis of the obtained sequencing data. Moreover, I provide sequencing input to other lab members and collaborators regarding their sequencing-based projects.
Career Goal:

My future career goal is to obtain a PhD in Immunology, a field of study in science which fascinates me, and, ultimately, to pursue a career in scientific research of immunological diseases.

Ryan Nelson

Visiting Scientist

Dr. Nelson was born and raised in Rockford, IL. As an undergraduate at Northwestern University, he gained exposure to medicine by volunteering at Children’s Memorial Hospital and also had his first research experience working in a lab investigating the effect of tRNA structure on translation. In medical school, he continued his research pursuits, participating in basic science investigation on the effect of dietary monosaccharides in the virulence of pseudomonas. There, he also completed a retrospective study characterizing the post-operative complications in ulcerative colitis patients undergoing colectomy after attempted medical rescue therapy. As a resident at Northwestern, his interest in pulmonary and critical care medicine began following an early ICU rotation. He then joined a lab studying potential therapeutics for influenza. Currently, Dr. Nelson is working with Dr. Akuthota investigating eosinophil biology in relation to severe asthma.

Veena Shivagouda Patil

Instructor

Biosketch:
I obtained MS in Genetics and Plant Breeding from UAS, Dharwad, India. Went to Temasek Life Sciences Laboratory (TLL), Singapore to do my Doctoral research with Dr Toshie Kai. Obtained my PhD (Biological Sciences) from SBS, NTU, Singapore in 2011. During doctoral research thesis, I worked on piRNAs in the Drosophila germline. I worked with Dr Tariq Rana (SBMRI/UCSD) for Postdoctoral research from 2011 to 2015. While I was in his lab, I worked to understand the role(s) of lncRNAs in innate immunity. In another project, I studies the role(s) of kinases in induced pluripotent stem cell (iPSC) generation. I joined Vijay’s group at LJI in 2015 to understand immunological responses in various infectious diseases such as Dengue and Tuberculosis using genomics and cell biological tools.

Benjamin Schmiedel

Instructor

Biosketch:
I obtained my Diploma in Biochemistry from University in Halle-Wittenberg (Germany) in 2006. Fascinated by human immunology and translational research, I undertook my PhD in tumor immunology at the University of Tuebingen (Germany). I joined the La Jolla Institute for Immunology (LJI) as a Postdoc in July 2012, and got appointed as Instructor in December 2016.

Research Focus:
In a pilot study, I focused on epigenetic mechanisms that modulate the expression of genes implicated in susceptibility and development of asthma, as well as the role of these genes for the function of human T cells. Subsequently, I’m managing a large-scale, genome-wide study on gene expression and epigenetic markers in various types of human immune cells, which will aid the understanding of epigenetic variations that cause the susceptibility to disease.

Career Goals:
I plan to pursue a career in scientific research focusing on the discovery of drug targets to improve the treatment options of human immunological diseases and cancer.

Greg Seumois

Core Director, Sequencing & Instructor

Biosketch:
Born and bred in Belgium, I obtained an MSc in Biology (1998 -University of Mons) and a PhD in Immunology (2007 – University of Liege). In 2006, I crossed the English Channel to go learn more about human immunology and translational research in Southampton (Pr. Djukanovitch Lab, UK). It’s in UK I met Vijay with who I started to work on an ambitious program of research on T cells epigenetics in asthma. Part of our epigenetic post-doc training, we joined Mark Ansel’s team at UCSF, in San Francisco. In 2010 we accepted the opportunity to be part of Anjana Rao’s research group here in LJI. In 2012, I got promoted LJI-Instructor. Recently I took Vijay’s challenge to be part, on his side, to the development of a laboratory of Immuno-genomics in LJI. Finally, involved in the development and day-to-day management since its creation in 2013, I’m Core Director of the LJI – Next Generation Sequencing Facility.

Research Focus:
I dedicate my time and effort to develop high-throughput sequencing technologies such as single cell transcriptomic, ChIP-Seq, ChIA-PET, and many more… The scientific rational is to understand better and with a different angle, the mechanisms of epigenetic regulation of gene expression in (rare) immune cells, pathologically relevant in many diseases such as asthma, SLE, tuberculosis…

Career Goals:
As an independent research group leader, I aim to develop innovative ways to investigate, understand and, ultimately, cure human diseases.

Divya Singh

Research Technician II

Biosketch:
I graduated from Johns Hopkins University, Baltimore with a Master’s degree in Biotechnology. I joined LJI in 2013 as a Research Technician. I am currently involved in RNA sequencing for two projects focused on transcriptome analysis of immune cells in various immunological diseases and cancer.

Career goals:
My goal is to become a scientific researcher involved in the genetic and functional studies of human diseases.

Ciro Ramirez Suastegui

Research Technician I

Biosketch:
I will graduate in September 2018 with a bachelor in Genomic Sciences from the National
Autonomous University of Mexico. This is an interdisciplinary program which combines mainly
biology, computer science, and mathematics. I joined La Jolla Institute for Allergy and
Immunology in June of 2017.

Research Focus:
I have worked on Evolution in mammals using bioinformatics, specially focused on sexual
chromosomes history. At LIAI I am involved in the analysis of transcriptomic data in various types of human immune cells and in different diseases.

Career Goals:
My goal is to become a bioinformatic tools developer and help the scientific community with
the software I create. I would focus on Single Cell software development, but I am open to
explore other fields, given than bioinformatics is so flexible.

Alice Wang

Research Tech I

Biosketch:
I obtained my M.S. Degree in Physiology 2017 in a joint program from George Mason University and Georgetown University. As a native San Diegan I really missed the beautiful weather here, so shortly after graduation, I came back to San Diego and joined the Vijayanand Lab. As a research technician, I assist with various activities including RNA sequencing projects for other lab members and collaborators. Every day, I continue to apply my knowledge in human physiology to further understand how the immune system responds to infectious and respiratory diseases.

Career Goal:
My goal is to become a scientific researcher focused on understanding how the immune system responds to human diseases in order to develop potential vaccines and novel treatments.

Vijayanand Lab

Research Projects

Asthma

The Vijayanand lab employs genomic and epigenomic tools to understand the molecular basis of asthma.

Selected References

Selected References

Seumois G, Chavez L, Gerasimova A, Lienhard M, Omran N, Kalinke L, Vedanayagam M, Ganesan AP, Chawla A, Djukanović R, Ansel KM, Peters B, Rao A, Vijayanand P. (2014). Epigenomic analysis of primary human T cells reveals novel enhancers associated with Th2 memory differentiation and asthma susceptibility. Nature Immunology 15(8):777-88. PMCID: PMC4140783

Schmiedel BJ, Seumois G, Samaniego-Castruita D, Cayford J, Schulten V, Chavez L, Ay F, Sette A, Peters B, Vijayanand P. (2016). 17q21 asthma-risk variants switch CTCF binding and regulate IL-2 production by T cells. Nature Communications. 7:13426. PMCID: PMC5116091.

Engel, I., Seumois, G., Chavez, L., Chawla, A., Castruita, D.S., White, B., Mock, D., Vijayanand, P*., Kronenberg, M*. (*joint senior authors) (2016). Innate-like functions of natural killer T cell subsets result from highly divergent gene programs. Nature Immunology. 17(6):728-39. PMCID: PMC4944658.

Seumois, G., Zapardiel-Gonzalo, J., White, B., Singh, D., Schulten, V., Dillon, M., Hinz, D., Broide, D.H, Sette, A., Peters, B., Vijayanand, P (2016). Transcriptional profiling of Th2 cells identifies pathogenic features associated with asthma. Journal of Immunology. PMCID: PMC4936908

Grants

NIH/NIAID, R01AI121426
Epigenome-wide association study of childhood asthma
Objective: Investigate the epigenetic basis of childhood asthma.
Role: Contact PI (P. Vijayanand)

NIH/NHLBI, R01HL114093
Epigenetics of severe asthma
Objective: To identify molecules that drive the pathogenesis of severe asthma.
Role: Contact PI (P. Vijayanand)

NIH/NHLBI, UG1HL139117
San Diego Team Asthma Management using Phenotypes (STAMP)
Objective: To develop personalized therapies for patients with severe asthma
Role: Multiple PI (P.Vijayanand, P. Akuthota, S. Jain)

NIH/NIAID U19AI135731
LJI Epitope Validation Center: Characterization of epitope-specific T cells responding to food, fungal and inner city allergens
Objective: Molecular characterization of fungal-allergen-epitope specific T cells in asthma.
Role: PI of Project 3

GENETICS OF HUMAN DISEASES (https://www.dice-database.org)

Selected References

Selected References

Benjamin J. Schmiedel, D. S., Ariel Madrigal, Alan G. Valdovino-Gonzalez, Brandie M. White, Jose Zapardiel-Gonzalo, Brendan Ha, Gokmen Altay, Jason A. Greenbaum, Graham McVicker, Grégory Seumois, Anjana Rao, Mitchell Kronenberg, Bjoern Peters, Vijayanand P. Impact of genetic polymorphisms on human immune cell gene expression. 2018 CELL (in press)

Schmiedel BJ, Seumois G, Samaniego-Castruita D, Cayford J, Schulten V, Chavez L, Ay F, Sette A, Peters B, Vijayanand P. (2016). 17q21 asthma-risk variants switch CTCF binding and regulate IL-2 production by T cells. Nature Communications. 7:13426. PMCID: PMC5116091.

Grants

NIH/NIAID, R24AI108564
Research resources: epigenomic and transcriptomic profiles of human immune

CANCER IMMUNOLOGY, TISSUE RESIDENT MEMORY T CELLS, CYTOTOXIC CD4 T CELLS

Selected References

Selected References

Ganesan AP, Clarke J, Wood O, Garrido-Martin E, Chee S, Mellows T, Samaniego-Castruita D, Singh D, Seumois G, Alzetani A, Woo E, Friedmann PS, King E, Thomas GJ, Sanchez-Elsner T, Vijayanand, P* & Ottensmeier, C. (*corresponding author) (2017). Tissue-resident memory features are linked to the magnitude of cytotoxic T cell responses in human lung cancer. Nature Immunology. 18 (8):940-950. PMID: 28628092

Patil V, Madrigal A, Schmiedel B, Clarke J, O’Rourke P, Silva A, Harris E, Peters B, Seumois G, Weiskopf D, Sette A, Vijayanand P. Precursors of human CD4+ cytotoxic T lymphocytes identified by single-cell transcriptome analysis. Science Immunology. 2018 Jan 19; 3(19): eaan8664 PMID: 29352091

Cancer Research UK Network Accelerator Award

Molecular analysis of the success determinants and mechanisms of solid tumour immunotherapy